See more information about “Cost and Reservation Details” on our Scheduling an Exploration page.
High School Explorations
DNA Science for High Schoolers
For the advanced gel electrophoresis portion there are several different labs available depending on your interests (all of these labs utilize the E-gel system):
We will discuss original fingerprinting methods and the technique commonly used today and made popular by “CSI” and similar shows. The students will have the opportunity to perform gel electrophoresis using DNA samples simulating a crime scene. Students will analyze their results and compare banding patterns to determine the guilty party. This activity is also available as a “Harry Potter” storyline.
Students receive a 24-member family tree and DNA samples presumably taken from each family member. They determine the genotype of each of the relatives by cutting the DNA samples with a restriction enzyme and separating the fragments by gel electrophoresis. Please note there is an additional $50 cost associated with this lab.
Students become virus hunters as they interpret DNA fingerprints to identify the viral strain responsible for a potentially deadly fictitious disease outbreak (uses harmless predigested DNA).
DNA Restriction Enzyme Analysis and Bacterial Transformation (AP Biology Investigations 8 & 9)
$375 DNA Restriction Enzyme Analysis
$325 Bacterial Transformation
AP Biology or Advanced students, Grades 10-12
- Pipetting by Design
- Restriction Digest and Analysis of Lambda DNA
- Advanced Gel Electrophoresis
- pGLO Bacterial Transformation
This exploration is designed in-line with the AP Biology Investigations 8 & 9. The restriction digestion or bacterial transformation can also be scheduled separately. Restriction enzymes are used by bacteria to cut DNA at specific nucleotide sequences. In the laboratory, these enzymes are often used as molecular scissors for genetic engineering. In this lab, students use three restriction enzymes (EcoRI, HindIII, and PstI) to cut DNA. Students then use agarose gel electrophoresis to separate the DNA fragments by size and analyze the DNA pattern on the gel. Bacteria can be genetically modified by transformation, a process whereby cells take up foreign DNA. In this laboratory, students induce bacteria to take up DNA-containing genes that give the bacteria two new characteristics: resistance to the antibiotic ampicillin and the ability to fluoresce green. Back in their classroom, students will verify the presence of these traits and determine their transformation efficiency.
This Exploration is available to advanced students in upper grades. Each laboratory includes 8 work stations with a maximum of 32 students. The DNA restriction enzyme and bacterial transformation laboratories can be completed on the same day. This exploration uses the E-Gel system. Students have the opportunity to visit the Reitz Union and UF Bookstore/Welcome Center for lunch.
Polymerase Chain Reaction (PCR)
Part 1 = 2 hours
Part 2 = 3.5 hours or overnight for thermal cycler to run
Part 3 = 1 hour
Advanced students, Grades 10-12
- Pipetting by Design
- PCR (there are several options available, please see below)
- Advanced Gel Electrophoresis
Students use real–world forensic techniques to extract DNA from their hair follicles or cheek cells, and then use PCR amplification and electrophoresis to fingerprint their own DNA at a specific genetic locus (chromosome 16). Using their own results, students can also test the Hardy–Weinberg equilibrium theory within their classroom population, then go online to compare their classroom results to genetic data of populations worldwide.
Current regulations in the US do not require that genetically modified foods be labeled as such. Using common grocery store items, students test samples of their favorite foods for the presence of modified genes.
Using PCR, students will amplify short tandem repeats in DNA samples and determine which suspect matches the DNA found at the crime scene.
Contact us to develop a custom PCR lab to identify a gene of interest within an organism of your choice.
Grades 9-12 (Recommended for advanced students)
Comparative Protein Profiler includes:
- Protein extraction from fish samples (can use other organisms if desired)
- Polyacrylamide Gel Electrophoresis (SDS-PAGE)
- Gel Staining/Destaining
Students employ protein electrophoresis, the most widely used technique in life science research, to study protein structure and function. Students generate protein profiles from the muscles of both distantly and closely related species of fish and, from their results, compare the different species’ profiles, construct cladograms (phylogenetic trees), and assign each organism a branch.
The Pompe Predicament (short and extended versions)
- DNA Structure to Function Science Take-Out Kit
- GAA colorimetric assay
Pompe disease affords the rare opportunity for students to consider multiple biological concepts and assemble them into a story. Instead of DNA taught separate from protein structure and function, these areas as well as enzymes, genetics and human disease are all taught together through the story of Pompe disease.
DNA Science for Middle Schoolers
Deoxyribonucleic acid is as much fun to say as it is to work with. To enhance their learning of the structure of DNA, students will construct candy DNA models that are a tasty treat for the ride back home! Students will also extract DNA from plant cells so they can visualize genetic material.