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Biotechnology Lab
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The Biotechnology Lab was led by Dr. Dan Brown. He was assisted by Ms. Tammie Mandell, Biological Scientist. The workshop was held at the Shands Research Facility which is one of the most renowned and respected Research Hospitals in the U.S. The teachers were divided into pairs and became very familiar with running and interpreting DNA gels, preparing PCR products, and analyzing Southern Blots. These techniques underlie much of current science from anthropology to forensics, to gene therapy, to environmental and agricultural science, to sheep cloning and drug design. The teachers learned about methods, approaches, applications and resources for their individual research assignments.
During the Biotechnology Lab we had the chance to get to know each other as professionals and share individual teaching experiences.
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Picture One: Restriction Enzyme Digestion of Bacteriophage DNA and Separation of Fragments by Gel Electrophoresis. HAEIII-restriction enzyme cuts DNA sequence "GGCC" HINDIII-enzyme cuts DNA at sequence "AAGCTT" |
Picture Two : Experiment 1 A Restriction Enzyme Digestion of Bacteriophage DNA samples Experiment 1 B Agarose Gel Electrophoresis |
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Picture Three : Detection of Restriction Fragment Length Polymorphisms in human DNA Introduction to Southern Blotting Exp. 2 Incremental Restriction Digestion of Han Genomic DNA Exp. 2 C Capillary Transfer of DNA to a Nylon Membrane |
Picture Four: Hybridization and Detection Chemilluminescent
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