Special Explorations for Teachers and Students (SETS)
DNA Restriction Enzyme Analysis
This exploration is designed in-line with the AP Biology Lab 6 curriculum. Restriction enzymes are used by bacteria to cut DNA at specific nucleotide sequences. In the laboratory, these enzymes are often used as molecular scissors for genetic engineering. In this lab, students use three restriction enzymes (EcoRI, HindIII, and BamHI or PstI) to cut DNA. Students then use agarose gel electrophoresis to separate the DNA fragments by size and analyze the DNA pattern on the gel. Site visits and guest speakers will be arranged as time allows.
This Exploration is available to advanced students in upper grades. Each laboratory includes 8 work stations with a maximum of 32 students. The DNA restriction enzyme and bacterial transformation laboratories can be completed on the same day. In lieu of the UF Experience exploration, the students will have the opportunity to visit the Reitz Union and UF Bookstore/Welcome Center for lunch.
**AP classes from different schools may try to coordinate visits on the same dates, provided the total number of students does not exceed 32.
The cost of this Exploration is $175.
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