Transformation

Special Explorations for Teachers and Students (SETS)

Bacterial transformation

This exploration is designed in-line with the AP Biology Lab 6 curriculum. Bacteria can be genetically modified by transformation, a process whereby cells take up foreign DNA. In this laboratory, students induce bacteria to take up DNA-containing genes that give the bacteria two new characteristics: resistance to the antibiotic ampicillin and the ability to fluoresce green. Back in their classroom, students will verify the presence of these traits and determine their transformation efficiency. Site visits and guest speakers will be arranged as time allows.

This Exploration is available to advanced students in upper grades. Each laboratory includes 8 work stations with a maximum of 32 students. The DNA restriction enzyme and bacterial transformation laboratories can be completed on the same day. In lieu of the UF Experience exploration, the students will have the opportunity to visit the Reitz Union and UF Bookstore/Welcome Center for lunch.

**AP classes from different schools may try to coordinate visits on the same dates, provided the total number of students does not exceed 32.

The cost of this Exploration is $125.

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   UF Experience
   Energy
   DNA Science I
   DNA Science II
   Anatomy and Physiology

Advanced Biotechnology ...
   DNA Restriction Enzyme Analysis
   Bacterial Transformation
   Human DNA Fingerprinting

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